Mitochondrial outer membrane permeabilization at the single molecule level
نویسندگان
چکیده
منابع مشابه
In vivo imaging of drug-induced mitochondrial outer membrane permeabilization at single-cell resolution.
Observing drug responses in the tumor microenvironment in vivo can be technically challenging. As a result, cellular responses to molecularly targeted cancer drugs are often studied in cell culture, which does not accurately represent the behavior of cancer cells growing in vivo. Using high-resolution microscopy and fluorescently labeled genetic reporters for apoptosis, we developed an approach...
متن کاملHow do BCL-2 proteins induce mitochondrial outer membrane permeabilization?
The mitochondrial pathway of apoptosis proceeds when molecules sequestered between the outer and inner mitochondrial membranes are released to the cytosol by mitochondrial outer membrane permeabilization (MOMP). This process is controlled by the BCL-2 family, which is composed of both pro- and anti-apoptotic proteins. Although there is no disagreement that BCL-2 proteins regulate apoptosis, the...
متن کاملIntegrated Systems and Technologies In Vivo Imaging of Drug-Induced Mitochondrial Outer Membrane Permeabilization at Single-Cell Resolution
Observing drug responses in the tumor microenvironment in vivo can be technically challenging. As a result, cellular responses tomolecularly targeted cancer drugs are often studied in cell culture, which does not accurately represent the behavior of cancer cells growing in vivo. Using high-resolutionmicroscopy and fluorescently labeled genetic reporters for apoptosis, we developed an approach t...
متن کاملWhere killers meet--permeabilization of the outer mitochondrial membrane during apoptosis.
Although mitochondria are usually considered as supporters of life, they are also involved in cellular death. Mitochondrial outer membrane permeabilization (MOMP) is a crucial event during apoptosis because it causes the release of proapoptotic factors from the mitochondrial intermembrane space to the cytosol. MOMP is mainly controlled by the Bcl-2 family of proteins, which consists of both pro...
متن کاملMass action at the single-molecule level.
We developed a system to reversibly encapsulate small numbers of molecules in an array of nanofabricated "dimples". This system enables highly parallel, long-term, and attachment-free studies of molecular dynamics via single-molecule fluorescence. In studies of bimolecular reactions of small numbers of confined molecules, we see phenomena that, while expected from basic statistical mechanics, a...
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ژورنال
عنوان ژورنال: Cellular and Molecular Life Sciences
سال: 2021
ISSN: 1420-682X,1420-9071
DOI: 10.1007/s00018-021-03771-4